Device for synthesis of radiopharmaceutical products

ABSTRACT

The invention concerns a device for synthesis of radiopharmaceutical products based on chemical reagents contained in bottles, said device comprising several reaction compartments, transfer means between said bottles and said reaction compartments as well as mechanical means acting on said transfer means and enabling to monitor and control mechanically the transfer of chemical reagents. The invention is characterized in that it comprises: a fixed module including at least the mechanical means; a removable and disposable module, essentially in the shape of a support, whereon are arranged the transfer means between said bottles and said reaction compartments, said removable and disposable module not including any mechanical means; and means for securing said removable and disposable module to said fixed module.

SUBJECT OF THE INVENTION

[0001] The present invention relates to a novel device for synthesis of radiopharmaceutical products from radioactive compounds.

[0002] The present invention relates more especially to the use of this device for synthesis of radiopharmaceutical products from reagents containing radioactive elements with a short half-life, such as ¹¹C, ¹³N, ¹⁵O or ¹⁸F.

PRIOR ART

[0003] In diagnostic applications, such as Positron Emission Tomography (PET), use is made of radiochemical compounds, also called radiopharmaceutical compounds, which are labelled by means of an element such as ¹¹C, ¹³N, ¹⁵O or ¹⁸F. One example of such compounds is 2-[¹⁸F]fluoro-2-deoxy-D-glucose, commonly called ¹⁸FDG.

[0004] Synthesis of these radiopharmaceutical compounds with a short half-life is performed in apparatuses that enable the different chemical compounds for carrying out the synthesis to be brought into contact and heated during the reaction and allow the products obtained to be purified.

[0005] To avoid any risk associated with the handling of radioactive substances, these apparatuses are placed in a shielded and monitored environment. These apparatuses are generally linked to an automaton which commands the various operations enabling the performance of this synthesis, comprising reaction and heating stages, which are associated with transfers of chemical reagents.

[0006] Owing to the short half-life of these radioisotopes that are used, it is necessary to perform this type of synthesis at regular intervals. This multiplies the risks of contamination from one synthesis to another, while the end product is intended for injection into patients.

[0007] Moreover, a certain number of materials contained in the components of the synthesis apparatus are particularly sensitive to radioactivity and/or to chemical corrosion. This is the case, for example, with the valves or tubes allowing transfers to take place.

[0008] It is therefore necessary to wash and sterilise each component of the synthesis apparatus at regular intervals, especially all the tubing and all the valves which allow transfer of the reagents, hence the need to use components that can be sterilised easily. Users must furthermore ensure thorough, careful and regular maintenance of all the components of the apparatus.

[0009] U.S. Pat. No. 5,759,513 discloses an apparatus and a process for preparing radiochemical compounds in which, with the exception of the reagent labelled by means of an element with a short half-life, the reagents required are metered into containers beforehand. This gives a kit containing the said containers, the reactors, the columns and the transfer tubes fitted with valves, which are required for the process. This kit comprises a large number of elements, including valves. The overall cost does not allow it to be used as a single-use kit.

[0010] WO-A-93/11871 discloses an apparatus for preparing radiochemical compounds, comprising a single-use module. This apparatus is intended to be connected to reagent containers and comprises means of metering these reagents constituted by injectors provided with pistons and actuated by stepping motors. Complex, expensive means that require handling by the operator are necessary to connect the stepping motors to the rods of the injectors.

[0011] U.S. Pat. No. 5,415,843 describes an apparatus for manufacturing radiopharmaceutical compounds which has certain characteristics in common with that described in WO-A-93/11871. This apparatus includes a single-use disposable kit which comprises a plate and receptacles but not the connection tubes between the various receptacles.

[0012] U.S. Pat. No. 5,312,592 also describes an apparatus which has certain characteristics in common with that described in WO-A-93/11871. This document relates more especially to a disposable kit that can be used in the said apparatus and comprises a plate provided with openings and sleeves to support liquid injectors and to connect transfer tubes, as well as a hydrolysis receptacle.

[0013] The article by Chaly T. et al., Appl. Radiat. Isot. Vol. 41, No. 1, pp 29-34 (1990) in Int. J. “Radiat. Appl. Instrum. Part A” (Pergamon Press) describes a process for producing ¹⁸FDG by using a synthesis unit comprising disposable sterile elements such as syringes, needles, tubes, adapters and stop valves.

OBJECTS OF THE INVENTION

[0014] It is an object of the present invention to provide a device for synthesis of radioactive products, in particular radiopharmaceutical products, from radioactive elements with a short half-life, which can be used easily.

[0015] In particular, it is an object of the present invention to provide a device which minimises the risks of contamination from one synthesis to another.

[0016] It is likewise an object of the present invention to provide a device which is easier to maintain and service.

[0017] In particular, it is an object of the present invention to provide a device which can be washed easily and sterilised easily.

BRIEF DESCRIPTION OF THE INVENTION

[0018] The present invention relates to a device for synthesis of radiopharmaceutical products from chemical reagents contained in bottles, the said device comprising several reaction compartments, transfer means between the said bottles and the said reaction compartments as well as mechanical means acting on the said transfer means and enabling to monitor and control mechanically the transfer of the chemical reagents, characterised in that it comprises:

[0019] a fixed module including at least the mechanical means,

[0020] a removable and disposable module, essentially in the shape of a support plate, on which the transfer means between the said bottles and the said reaction compartments are arranged, the said disposable and removable module not including any mechanical means, and

[0021] means for securing the said removable and disposable module to the said fixed module.

[0022] The said disposable module preferably furthermore comprises the said bottles and the said reaction compartments.

[0023] The bottles are preferably pre-metered bottles.

[0024] The support plate preferably comprises means for the precise positioning of the transfer means.

[0025] The positioning means are preferably constituted by grooves in which the transfer means are placed.

[0026] The transfer means are preferably flexible tubes, the mechanical means are pistons, the transfer means, the mechanical means and the positioning means being capable of co-operating so as to form valves.

[0027] The securing means preferably comprise at least one opening, shoulders and fasteners.

[0028] The said shoulders are preferably located on the support plate, preferably at the four corners of the support plate, and are capable of fitting into fasteners located on the fixed module.

[0029] The said opening is preferably likewise located on the support plate, preferably at the centre of the said plate, and is capable of fitting into a fastener located on the fixed module.

[0030] The nature of the support plate (5) and that of the transfer means are preferably such that they allow washing of the said disposable module by steam autoclaving and/or its sterilisation.

[0031] The support plate is preferably made of ABS and the transfer means are preferably made of silicone.

[0032] The device preferably includes means of actuating the mechanical means of the fixed module.

[0033] The actuating means are preferably constituted by an automaton co-operating with a computer and forming part of the fixed module.

[0034] The present invention likewise relates to a disposable and removable module intended for association with a fixed module by securing means and essentially in the form of a support plate comprising means for positioning the means for transferring chemical reagents.

[0035] The said module preferably furthermore comprises bottles and reaction compartments.

[0036] The said module preferably likewise comprises columns.

[0037] The positioning means are preferably constituted by grooves capable of receiving transfer means.

[0038] The present invention likewise relates to a process for synthesising radiopharmaceutical products using the device according to the present invention and comprising the following stages:

[0039] the transfer means are arranged on the support plate,

[0040] the bottles containing the chemical reagents are fixed to one of the ends of the transfer tubes,

[0041] the said support plate with the transfer means is attached to the fixed module with the aid of securing means,

[0042] the transfer means are connected to the various elements of the fixed module,

[0043] the various operations required for synthesis of the pharmaceutical products, including the actuation of the mechanical means, are commanded via the automaton.

[0044] Before commanding the various operations required for synthesis of the pharmaceutical products, a test to verify the tightness of the device is preferably carried out via the automaton.

[0045] The present invention also relates to the use of the device according to the present invention or of the module according to the present invention for synthesising radiopharmaceutical compounds containing radio-elements with a short half-life, preferably ¹¹C, ¹³N, ¹⁵O or ¹⁸F.

BRIEF DESCRIPTION OF THE FIGURES

[0046]FIG. 1 provides a general view of the disposable module fixed on the fixed module of the device according to the present invention.

[0047]FIG. 2 represents a perspective view of the fixed module in the device according to the present invention.

[0048]FIG. 3 represents a perspective view of the front face of the disposable module without the fixed module in the device according to the present invention.

[0049]FIG. 4 represents a perspective view of the rear face of the disposable module without the fixed module in the device according to the present invention.

[0050]FIG. 5 shows the mechanism for closing the flexible tubes belonging to the disposable module by means of pistons belonging to the fixed module according to a preferred embodiment of the invention.

DETAILED DESCRIPTION OF A PREFERRED EMBODIMENT OF THE INVENTION

[0051] It should be noted that the figures presented here relate to a device intended for synthesising ¹⁸FDG. These figures are given purely by way of example to illustrate the invention, but they do not in any way limit its scope. The person skilled in the art will easily be able to adapt the various elements of the device depending on the type of synthesis to be performed.

[0052] As illustrated in FIG. 1, the device according to the present invention comprises a fixed module 1 and a removable and disposable single-use module 2. When the device is in operation, the removable and disposable module 2 is fixed on the fixed module 1.

[0053]FIG. 2 provides a detailed view of the fixed module. The fixed module 1 is here in the form of a case 7, on which pistons 8 a,8 b,8 c,8 e, . . . are arranged.

[0054] The module 1 likewise comprises an automaton controlled by a computer, although these are not shown here. The automaton controls all the operations required to implement synthesis of the radiopharmaceutical products, in particular the movement of the pistons 8 a,8 b,8 c,8 d,8 e, . . .

[0055] Moreover, to enable the device to be used, the module 1 comprises securing means which enable it to be fixed to the disposable module 2. These securing means can take the form of fasteners arranged according to a precise configuration. In the case shown in FIG. 2, these fasteners are of two types. There are, on the one hand, the four fasteners 12 a,12 b,12 c and 12 d and the central fastener 11, the said central fastener 11 here being in the form of a stud. The presence of the central fastener 11 is intended for securing the fixing of the module 2 to the module 1. It furthermore allows the pressure exerted by the pistons 8 a,8 b,8 c,8 d,8 e, . . . to be counteracted.

[0056]FIGS. 3 and 4 give a more detailed view of the various elements of the disposable module according to the present invention. The disposable module 2 comprises at least:

[0057] one plate 5, in which a network of grooves 6 is formed according to a precise configuration;

[0058] five bottles 3 a,3 b,3 c,3 d,3 e containing the pre-metered chemical reagents,. respectively Kryptofix, K₂CO₃, acetonitrile, triflate, water and sodium hydroxide;

[0059] tubes 4 a,4 b,4 c,4 d,4 e, . . . for circulating the chemical products within the synthesis device, these tubes being flexible in the present case, and one of their ends being connected to the bottles 3 a,3 b,3 c,3 d,3 e, respectively,

[0060] columns, including outlet columns (not shown). The module 1 here comprises at least four columns, column C₁ as shown in FIG. 3 and columns C₂, C₃, C₄, not shown,

[0061] reaction compartments R₁ and R₂, as shown in FIG. 1.

[0062] The said plate 5 has a dual function. Its first function is that the plate 5 serves as a support for the tubes 4 a,4 b,4 c,4 d,4 e when these tubes are fixed in the grooves 6. The configuration of these grooves 6 allows very accurate positioning of the tubes 4 a,4 b,4 c,4 d,4 e relative to one another. In other words, the tubes 4 a-4 e, the pistons 8 a-8 e and the grooves 6 are capable of co-operating so as to form valves. The pistons 8 a-8 e associated with the portion of the tubes 4 a-4 e which face them thus form valves of the “pinch-valve” type, that is to say piston valves.

[0063] It is thus important that the tubes should be positioned correctly to enable these valves to operate correctly. In particular, the pistons 8 a-8 e should be able to act precisely on the said tubes 4 a-4 e.

[0064] The second function of the plate 5 is to serve as an interface between the fixed module 1 and the disposable module 2. The plate 5 is provided with securing means which enable the said disposable module 2 to be fixed on the said fixed module 1. By virtue of their shape, these securing means located on the disposable module 2 are complementary to the securing means located on the fixed module 1.

[0065] According to the exemplary embodiment shown in FIGS. 3 and 4, these securing means on the one hand take the form of shoulders 13 a,13 b,13 c,13 d, each located at one corner of the plate 5. These shoulders 13 a,13 b,13 c,13 d are capable of fitting into the respective fasteners 12 a,12 b,12 c,12 d. On the other hand, the securing means take the form of an opening 10 which is located in the centre of the plate 5 and into which the central fastener 11 of the fixed module 1 can fit. By virtue of its central position in the plate 5, the opening 10 makes it possible to ensure that the disposable module 2 is secured in an optimum manner to the fixed module 1 while the device is being used.

[0066] However, the securing means can take other forms.

[0067]FIG. 5 illustrates how the pistons 8 a-8 e located on the fixed module 1 bring about either the opening or closure of the tubes 4 a-4 e of the disposable module 2 as a function of the movement that they are commanded to make by the automaton. In view a, this figure shows the head 9 of one of these pistons 8 a and a section of one of the flexible tubes 4 a in an open position, that is to say when the tube 4 a is open. In this position, the head 9 of the piston 8 a is disengaged from the tube 4 a and transfers of chemical products are then allowed. When commanded by the automaton, on the other hand, the head 9 of the piston 8 a is displaced and comes to exert a pressure on the section of the tube 4 a, as illustrated in view b, and the tube 4 a is then closed, preventing any transfer of chemical products.

[0068] In relation to the prior-art devices, the present invention has the original characteristic of being without any mechanical means, in particular mechanical means of opening and closing the tubes, these mechanical means being located on the fixed module 1.

[0069] By virtue of this fact, the module 2 can be attached and removed easily. It can be fixed easily on the fixed module 1. Such a module 2 likewise has the advantage of being inexpensive, owing especially to the absence of mechanical means in this module, and, by virtue of this fact, allows single use.

[0070] According to a preferred embodiment, the plate 5 is made of ABS and the tubes 4 a,4 b,4 c,4 d,4 e, . . . are made of silicone. The advantage of such a choice in terms of the materials used is that these elements are capable of withstanding the high pressures, in particular the pressures exerted by the pistons, during the use of the device. It likewise makes it possible to limit the waste resulting from incineration of used disposable modules.

[0071] Insofar as their composition allows, the various elements constituting this module can be sterilised easily, in particular by the conventional techniques such as sterilisation by gamma radiation.

[0072] The example described below is an example of the use of the device according to the present invention for synthesis of ¹⁸FDG. However, this example is given only by way of illustration. Other uses are possible.

[0073] To synthesise ¹⁸FDG with the device according to the present invention, ¹⁸F⁻ is first of all extracted from the mixture H₂ ¹⁸O—H₂O—¹⁸F⁻. To do this, the mixture is transferred by means of gaseous helium as far as the column C₁ containing an anion exchange resin, such as QMA Waters™. Column C₁ allows recovery of the ¹⁸F⁻, while H₂ ¹⁸O—H₂O is transferred to the reaction compartment R1.

[0074] Tube 4 e is opened to allow 0.75 ml of a solution of K₂CO₃/K2.2.2/H₂O/CH₃CN contained in bottle 3 e to be transferred through column C1 with the aid of a vacuum pump in a reaction compartment or reactor R₂. The ¹⁸F⁻ is thus eluted, and it is transferred to reaction compartment R₂.

[0075] The temperature of the reactor R₂ is increased to 90° C. and H₂O is eliminated by forming an azeotrope with acetonitrile.

[0076] Tube 4 d is then opened, and 0.5 ml of acetonitrile contained in bottle 3 d is then transferred with the aid of a vacuum pump in reaction compartment R2.

[0077] The traces of H₂O are eliminated by forming an azeotrope with acetonitrile. The evaporation is continued to complete the drying.

[0078] Tube 4 c is opened so as to transfer with the aid of a vacuum pump a solution of 13 mg of 1,3,4,6-tetra-O-acetyl-2-O-trifluoromethane-sulphonyl-β-D-mannopyranose in 1 ml of acetonitrile contained in bottle 3 c into compartment R₂. The temperature of the said compartment R₂ is brought to 85° C.

[0079] The acetonitrile is then evaporated in vacuo while maintaining the temperature in the reactor R₂ between 90 and 85° C.

[0080] Tube 4 b is then opened so as to transfer 1 ml of H₂O contained in bottle 3 b with the aid of a vacuum pump. The reactor R₂ is then cooled.

[0081] Tube 4 a is then opened so as to transfer 1 ml of a solution of 0.5 N NaOH contained in bottle 3 a to reactor R₂ with the aid of a vacuum pump.

[0082] Nitrogen at a pressure of 400 mbar is used to transfer the contents of reactor R₂ through purification columns C₂, C₃, C₄ (not shown) arranged in series at the outlet of the circuit.

[0083] Tube 4 b is opened for a second time to transfer 4 ml of H₂O from bottle 3 b to reactor R₂ with the aid of a vacuum pump.

[0084] Nitrogen at a pressure of 400 mbar is used a second time to transfer the contents of reactor R₂ through purification columns C₂, C₃, C₄.

[0085] Finally, it will be noted that the device according to the present invention offers the advantage of being particularly compact, thus reducing the volume of the shielded chamber in which it is used and hence the quantity of lead. 

1. Device for synthesis of radiopharmaceutical products from chemical reagents contained in bottles (3 a,3 b,3 c,3 d,3 e), the said device comprising several reaction compartments (R₁,R₂), transfer means (4 a,4 b,4 c,4 d,4 e, . . . ) between the said bottles (3 a,3 b,3 c,3 d,3 e) and the said reaction compartments (R₁,R₂) as well as mechanical means (8 a,8 b,8 c,8 d,8 e, . . . ) acting on the said transfer means (4 a,4 b,4 c,4 d,4 e, . . . ) and enabling to monitor and control mechanically the transfer of the chemical reagents, characterised in that it comprises: a fixed module (1) comprising at least the mechanical means (8 a,8 b,8 c,8 d,8 e, . . . ), a removable and disposable module (2), essentially in the shape of a support plate (5), on which the transfer means (4 a,4 b,4 c,4 d,4 e, . . . ) between the said bottles (3 a,3 b,3 c,3 d,3 e) and the said reaction compartments (R₁,R₂) are arranged, the said removable and disposable module (2) not including any mechanical means, and means (10, 11, 12 a-12 d, 13 a-13 d) for securing the said removable and disposable module (2) to the said fixed module (1).
 2. Device according to claim 1, characterised in that the said disposable module (2) furthermore comprises the said bottles (3 a,3 b,3 c,3 d,3 e) and the said reaction compartments (R₁,R₂).
 3. Device according to claim 1 or 2, characterised in that the bottles (3 a,3 b,3 c,3 d,3 e) are pre-metered bottles.
 4. Device according to any of the preceding claims, characterised in that the support plate (5) comprises means for the precise positioning of the transfer means (4 a,4 b,4 c,4 d,4 e, . . . ).
 5. Device according to claim 4, characterised in that the positioning means are constituted by grooves (6) in which the transfer means (4 a,4 b,4 c,4 d,4 e, . . . ) are placed.
 6. Device according to one of claims 1 to 5, characterised in that the transfer means are flexible tubes (4 a,4 b,4 c,4 d,4 e, . . . ), the mechanical means (8 a,8 b,8 c,8 d,8 e, . . . ) are pistons, the transfer means, the mechanical means and the positioning means (6) being capable of co-operating so as to form valves.
 7. Device according to any of claims 1 to 6, characterised in that the securing means comprise at least one opening (10), shoulders (13 a-13 d) and fasteners (11, 12 a-12 d).
 8. Device according to claim 7, characterised in that the said shoulders (13 a-13 d) are located on the support plate (5), preferably at the four corners of the support plate (5), and are capable of fitting into fasteners (12 a-12 d) located on the fixed module (1).
 9. Device according to claim 8, characterised in that the said opening (10) is likewise located on the support plate (5), preferably at the centre of the said plate (5), and is capable of fitting into a fastener (11) located on the fixed module (1).
 10. Device according to one of the preceding claims, characterised in that the nature of the support plate (5) and that of the transfer means (4 a,4 b,4 c,4 d,4 e) are such that they allow washing of the said disposable module (2) by steam autoclaving and/or its sterilisation.
 11. Device according to claim 10, characterised in that the support plate (5) is made of ABS.
 12. Device according to claim 10 or 11, characterised in that the transfer means (4 a,4 b,4 c,4 d,4 e, . . . ) are made of silicone.
 13. Device according to any of the preceding claims, characterised in that it includes means of actuating mechanical means of the fixed module (1).
 14. Device according to any of the preceding claims, characterised in that the actuating means are constituted by an automaton co-operating with a computer and forming part of the fixed module (1).
 15. Disposable and removable module (2) intended for association with a fixed module (1) by securing means (10, 11, 12 a-12 d, 13 a-13 d) and essentially in the form of a support plate (5) comprising means for positioning the means (4 a,4 b,4 c,4 d,4 e, . . . ) for transferring chemical reagents.
 16. Module (2) according to claim 15, characterised in that it furthermore comprises bottles (3 a,3 b,3 c,3 d,3 e) and reaction compartments (R₁,R₂).
 17. Plate according to claim 15 or 16, characterised in that the positioning means are constituted by grooves (6) capable of receiving transfer means (4 a,4 b,4 c,4 d,4 e).
 18. Process for synthesising radiopharmaceutical products using the device according to any of claims 1 to 14, characterised in that it comprises the following stages: the transfer means (4 a,4 b,4 c,4 d,4 e) are arranged on the support plate (5), the bottles (3 a-3 e) containing the chemical reagents are fixed to one of the ends of the transfer tubes (4 a,4 b,4 c,4 d,4 e), the said support plate (5) with the transfer means is attached to the fixed module (1) with the aid of securing means (10, 11, 12 a-12 d, 13 a-13 d), the transfer means (4 a,4 b,4 c,4 d,4 e) are connected to the various elements of the fixed module (1), the various operations required for synthesis of the pharmaceutical products, including the actuation of the mechanical means (8 a-8 e), are commanded via the automaton.
 19. Process according to claim 18, characterised in that, before commanding the various operations required for synthesis of the pharmaceutical products, a test to verify the tightness of the device is carried out via the automaton.
 20. Use of the device according to any of claims 1 to 14 or of the disposable module according to one of claims 15 to 17 for synthesising radiopharmaceutical compounds containing radio-elements with a short half-life, preferably ¹¹C, ¹³N, ¹⁵O or ¹⁸F. 